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Antimicrobial activity of non-bond colicin on candida albicans biofilm(Article)(Open Access)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Antimicrobial activity of non-bond colicin on candida albicans biofilm(Article)(Open Access) Authors: اسماعيل ابراهيم لطيف, Latif, Ismail Ibrahim Abstract: Two hundred fifty mid-stream urine specimens were collected from Baqubah Teaching Hospital and Al-Batool Teaching Hospital from patients with urinary tract infections (UTI). Of these investigated urine specimens, 66 (26.4%) specimens showed positive growth culture of Gram-negative bacteria. From these, Escherichia coli was the most prevalent bacteria of the examined culture (41, 62.12%). Additionally, the cup assay was used to determine colicin producers while the most efficient colicin producers were estimated by the formation of larger inhibition zone. Approximately half of the investigated E. coli isolates (20, 49 %) was colicin producers. Colicins was extracted after induction by mitomycin-C showed a concentration of 3020 μg/ml, as estimated utilizing the Lowry method, while its activity was 80 U/ml. Our study results showed that colicin had significant antibiofilm activity (P≤ 0.05) against Candida albicans and the effect seemed to be concentration dependent. . However, the values of biofilm inhibition varied depending on the different tested isolates. The biofilm of isolate 5 showed the most significant inhibition (P≤ 0.05) by colicin with a value of 46%, while isolate 3 was less affected with an inhibition rate of 19% at the concentration of 2500 μl/ml. © 2020 University of Baghdad-College of Science. All rights reserved.

New Bioactive Aromatic Indole Molecules with Monosaccharide Core

Sun, 01 Jan 2023 00:00:00 GMT

Title: New Bioactive Aromatic Indole Molecules with Monosaccharide Core Authors: صالح مهدي سلمان, Salman, Salih Mahdi Abstract: 1,1,2-trimethyl-1H-benzo[e]indole is an important heterocyclic compound, its available in reasonable price and can easily modified to make a good intermediate for other derivatives. That is quite enough reasons to use as starting material for a new series of compounds with other biomolecules such as monosaccharides after simple modification. The target molecules show biological activity. So, the current work is aiming to improve the activity and the properties of the benzo indole by attaching with a naturally occurring, and biodegradable compounds represented by 2-deoxy-2-amino -d-glucose and 6-deoxy-6-amino-d-glucose to synthesis both mono and di-saccharides derivatives of benzo indole. Two steps synthesis were used for mono-saccharide derivatives and three steps for di-saccharide derivatives, the first is the functionalization of 1,1,2-trimethyl-1H-benzo[e]indole [1] via the reaction with POCl3 to produce 2-(1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene) malonaldehyde [2] with two aldehydes reaction centers, while in the second step the latter was coupled with sugar via amino groups to get the two monosaccharide derivatives [3,5], while the disaccharides molecules [4,6] taken one more step with harder conditions to overcome the steric hindrance at the other reaction center. The purity and characterization of the target molecules was confirmed using spectroscopy methods including 1H NMR and 13 NMR. The synthesized compound shows a good biological activity as antibacterial antifungal.

The Effect of Different Types of Essential Oils on Some Blood Characteristics of Awassi Lambs(Article)(Open Access)

Mon, 01 Jan 2024 00:00:00 GMT

Title: The Effect of Different Types of Essential Oils on Some Blood Characteristics of Awassi Lambs(Article)(Open Access) Authors: بشار ادهم احمد, Ahmed, Bashar Adham Abstract: This study was conducted in the fields of the College of Veterinary Medicine - Tikrit University. The study included 20 Awassi lambs, 5 lambs for each treatment (T1 control, T2 using laurel oil, T3 using clove oil, T4 using sage oil) at the age of 6 months, dosed daily with a concentration of 500 mg/animal of the mentioned oils for a period of 90 days. This study is aimed to evaluate the effect of essential oils on blood characteristics (total protein, albumin, glucose, urea, cholesterol, triglycerides, uric acid, creatinine, calcium, magnesium, GOT, GPT, HDL, VDL, LDL, ALP).The results showed that using sage and clove oil was superior to the control treatment in terms of glucose (82.40, 80.40, 67.20, 56.80mg/dl)and triglycerides (91.40, 63.40, 60.20, 59.60 mg/dl), and adding clove oil was superior to the control treatment in calcium (9.81, 9.20, 9.32, 8.32 mg/dl) and magnesium (5.62, 5.40, 4.66, 4.56 mg/dl) and GPT (28.60, 24.80, 22.0, 21.80 U/L) and HDL (29.60, 26.60, 25. 40, 24.20 mg/dl). The laurel oilusing treatment was superior to the control in VDL (17.60, 16.20, 16.0, 14.80 mg/dl). © 2024 Indian Veterinary Assocaition. All rights reserved.

Genetic polymorphism in HSP90AA1 gene and associated with the heat tolerance coefficient in Holstein cows at south of Iraq(Article)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Genetic polymorphism in HSP90AA1 gene and associated with the heat tolerance coefficient in Holstein cows at south of Iraq(Article) Authors: صالح حسن جاسم, Jasim, salih hassan Abstract: This study was carried out in Iraq at the Taj Al-Nahrain Animal Production located in Diwaniyah province, one of the governorates of the Euphrates Middle, located south of the Iraqi capital Baghdad, about 180 kilometers away. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of HSP90AA1 gene with some of reproductive and productive traits and all so heat tolerance coefficient during different season (warm, moderate, cold) Here are the most important results obtained during this study. The analysis revealed a site of heterogeneity in the HSP90AA1 gene within the Exon3 region at site 1690. The genotype of the heterogeneity site was recorded with two genotypes: TT (Wild) and genotype TC) Heterozygous), and the heterogeneity of the nitrogen bases was determined. The results showed that the nitrogenous base of T was changed and replaced with the nitrogenous base C Within the heterogeneity site, the results show a significant difference at the level (P0<0.01) between the genotypes. Results have indicated in terms of the effect of genotypes For the studied piece For the site of heterogeneity and its relation to some reproductive qualities and productive qualities and heat tolerance coefficient To the absence of statistically significant differences between genotypes. © 2020 Plant Archives. All rights reserved.