DSpace Collection: كل نتاجات السادة التدريسين في جامعة ديالى لعام 2021

Antimicrobial activity of non-bond colicin on candida albicans biofilm(Article)(Open Access)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Antimicrobial activity of non-bond colicin on candida albicans biofilm(Article)(Open Access) Authors: اسماعيل ابراهيم لطيف, Latif, Ismail Ibrahim Abstract: Two hundred fifty mid-stream urine specimens were collected from Baqubah Teaching Hospital and Al-Batool Teaching Hospital from patients with urinary tract infections (UTI). Of these investigated urine specimens, 66 (26.4%) specimens showed positive growth culture of Gram-negative bacteria. From these, Escherichia coli was the most prevalent bacteria of the examined culture (41, 62.12%). Additionally, the cup assay was used to determine colicin producers while the most efficient colicin producers were estimated by the formation of larger inhibition zone. Approximately half of the investigated E. coli isolates (20, 49 %) was colicin producers. Colicins was extracted after induction by mitomycin-C showed a concentration of 3020 μg/ml, as estimated utilizing the Lowry method, while its activity was 80 U/ml. Our study results showed that colicin had significant antibiofilm activity (P≤ 0.05) against Candida albicans and the effect seemed to be concentration dependent. . However, the values of biofilm inhibition varied depending on the different tested isolates. The biofilm of isolate 5 showed the most significant inhibition (P≤ 0.05) by colicin with a value of 46%, while isolate 3 was less affected with an inhibition rate of 19% at the concentration of 2500 μl/ml. © 2020 University of Baghdad-College of Science. All rights reserved.

Genetic polymorphism in HSP90AA1 gene and associated with the heat tolerance coefficient in Holstein cows at south of Iraq(Article)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Genetic polymorphism in HSP90AA1 gene and associated with the heat tolerance coefficient in Holstein cows at south of Iraq(Article) Authors: صالح حسن جاسم, Jasim, salih hassan Abstract: This study was carried out in Iraq at the Taj Al-Nahrain Animal Production located in Diwaniyah province, one of the governorates of the Euphrates Middle, located south of the Iraqi capital Baghdad, about 180 kilometers away. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of HSP90AA1 gene with some of reproductive and productive traits and all so heat tolerance coefficient during different season (warm, moderate, cold) Here are the most important results obtained during this study. The analysis revealed a site of heterogeneity in the HSP90AA1 gene within the Exon3 region at site 1690. The genotype of the heterogeneity site was recorded with two genotypes: TT (Wild) and genotype TC) Heterozygous), and the heterogeneity of the nitrogen bases was determined. The results showed that the nitrogenous base of T was changed and replaced with the nitrogenous base C Within the heterogeneity site, the results show a significant difference at the level (P0<0.01) between the genotypes. Results have indicated in terms of the effect of genotypes For the studied piece For the site of heterogeneity and its relation to some reproductive qualities and productive qualities and heat tolerance coefficient To the absence of statistically significant differences between genotypes. © 2020 Plant Archives. All rights reserved.

Effect of the supplementation of probiotic and enzymes in laying hens diet on the eggs quality traits(Article)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Effect of the supplementation of probiotic and enzymes in laying hens diet on the eggs quality traits(Article) Authors: مهدي صالح جاسم, Jasim, MAhdi Salih Abstract: This study aimed to determine the effect of supplementation probiotics, digestive enzymes and synthesis of them into laying hens diet on egg quality traits. The field experiment lasted 74 days, including the duration of adaptation with 144 laying hens. Hens in the first treatment were fed on a standard layer diet (control), while the second third treatment on a standard layer diet with an addition of 0.5 and 0.75 g/ kg probiotic digestive enzymes in the treatment fed on standard layer diet. The fourth consists of standard diet with 0.5 g/ kg probiotic and 0.75 g/ kg digestive enzymes. The results showed an improvement in the quality characteristics of eggs produced from fed chickens for each of supplementation treatments significant increase in weight of eggs and shell and albumin. The probiotic treatment, synthesis treatment of probiotics and digestive enzymes led to significant improvement in shell thickness and yolk index. This concludes that the supplementation of the probiotic or digestive enzymes has improved the quality of the eggs produced. © 2021 Ecological Society of India. All rights reserved.

Effect of using antioxidants in chicken semen extender on stored semen traits(Article)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Effect of using antioxidants in chicken semen extender on stored semen traits(Article) Authors: خالد حامد حسن, Hassan, Khalid Hamid Abstract: The study was conducted to investigate the effect of adding various antioxidants to Lake diluent. The diluents used in the study were Lake (control), Lake plus taurine, Lake plus white tea and Lake plus trehalose, to dilute chicken semen and record their effect on semen quality traits. 14 males and 48 females of broiler breeder Ross 308 at 30 weeks of age were used in the experiment. The males were evaluated for sperm quality traits and selected for sperms concentration in semen to establish two genetic groups for highest sperms concentration and the lowest sperms concentration. A pool sample collected from each group separately and the pool sample divided into four parts each part diluted with different diluent and storage at 0°C for 2, 48, 72, 120 hours. The measurements were recorded for the following traits: individual motility, dead sperm percentage, abnormal sperm percentage and pH of semen after storage periods. The results showed significant differences between genetic groups in the individual motility trait. The Lake plus Taurine diluent recorded significant lower abnormal sperms percentage compared with other diluents. While the pH recorded significant higher value compared with trehalose. The storage period 2h had significant higher individual motility compared with others, while the abnormal sperms percentage at 2, 48 and 72 hours were low compared with 120 hours. There were significant interaction between taurine and genetic groups abnormal sperms percentage. © 2021