Determining the effect of various physicochemical factors on inhibition activity of Lactobacillus acidophilus against Klebsiella pneumonia isolated from hospital-acquired infections by using optical density assay

Abstract

This study aims to determine the effect of using Lactobacillus acidophilus in various physicochemical factors to inhibit the growth of Klebsiella pneumoniae isolated from patients of hospital-acquired infections by using optical density technique. The result revealed that different factors like pH, incubation temperature degree, incubation periods and concentrations, seemed to affect on inhibition activity of L. acidophilus. Maximum activity was noted at pH 3, temperature 45°C, incubation for 48 hr and three fold concentrations which clarified by values O.D reached to 0.111, 0.775, 0.660 and 0.040 respectively whereas the lowest effect appeared after the use of pH 9, temperature 15°C, incubation for24 hr and one fold concentrations where the inhibition values reached to 1.899, 1.180, 1.161 and 1.411respectively The results ensured that L. acidophilus sufficiency inhibited growth of K. pneumoniae with promising encourage to use the L.acidophilus as biotherapeutic agents against K. pneumoniae infections.