Relationship Between Oxidative Stress with Seminal Plasma Copper levels in Diyala infertile Men

Abstract

Objectives: The objective of the present study was to examine the published literature on the role of copper and malondialdehyde levels in seminal plasma of oligospermic, with normopermic men.Methods: The present study was conducted at Baquba Medical Hospital and a few commercial pathological laboratories in Diyala.. The patients attended the clinical with the complaint of infertility and were the male partner of married couples. The study was conducted from November 2011 to January 2012. And the infertility clinics were visited regularly to collect samples and relevant information of infertile subjects over the period at this time. 100 patients who fulfilled the inclusion criteria were selected for the study. Male subjects (23-42) years age with a mean±SDS=32.4±4.4 years There were 20 patients with oligospermia. Another twenty (25) fertile Iraqi men within a similar age group were studied as control. The control samples were treated similarly as the test samples. Copper in sample was assayed by using an atomic absorption spectrophotometric technique. Malondialdehyde was measured calorimetrically using thiobarbituric acid assay which detects thiobarbituric acid reactive substances.