MOLECULAR DETECTION OF erm(A), mef(A) IN Staphylococcus spp AND Streptococcus spp RESISTANT TO MACROLIDE FROM DIFFERENT CLINICAL INFECTIONS

Abstract

Isolates Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus albus, Streptococcus Pyogenes and Streptococcus agalactiae isolated from different clinical infections were activated. Results of susceptibility test showed that Staphylococcus aureus have resistance to antibiotics of erythromycin, azithromycin and clindomycin with percentage 45%, 45% 25% respectively, while Staphylococcus epidermidis resistant to erythromycin, azithromycin and clindomycin with ratio 66.6%, 44%, 22.2%, respectively. The resistance of erythromycin and azithromycin by Streptococcus pyogenes reached 25% and 37.5% respectively, whereas all isolates sensitive to clindomycin. The outcome disclosed that the values of minimum inhibitory concentrations of erythromycin ranged between 32 - ≥ 64 μg/ mL for all isolates. The results outcomes to 13(65%) isolates of Staphylococcus aureus produced Staphylokinase, 6(66.6%) isolates of Staphylococcus epidermidis able to produce the same enzyme. The results proved that 3(37.5%) isolates of Streptococcus pyogenes able to produce Streptokinase enzyme, but Streptococcus agalactiae could not produce streptokinase. The PCR-based assay, gene was detected in erm (A) gene was detected in 33.3% isolate of Staphylococcus spp. and 50% of Streptococcus spp, whereas mef (A) was detected in 46.6 % isolates of Staphylococcus spp. and 50% of Streptococcus spp .These findings demonstrated the variable phenotypic expression when compared to genotypic detection .