Improved Natural enrichment and differential medium for identification of Gram negative bacteria

Abstract

Because of the upmost interest in isolation and diagnosis of pathogenic and non pathogenic bacterial species in all over the world especially in the development country effers were made to synthesize new natural media for enriched and diagnosis of gram negative bacterial species. The new medium was prepared using common reed extract (Phragmites australis) and named IPA (Iraqi phragmites australis). The media were prepared at six recepies (IPA1, IPA2, IPA3, IPA4, IPA5, IPA6,). The components of the media are available localy and found quite efficient in enriched and differentiate between gram negative bacteria, when compared with media know to be suitable for such uses (Nutrient broth and Macconkey agar and blood agar). The results showed non significant differences between improved media and the control media. The new medium was un expensive and easy to prepare and efficient to others. This will make it a good substitution for many commercial media