Test of the effectively of some plant extracts against casuals of seedlings damping - off Pythium aphanidermatum (Edson) Fitzpatrick on cucumber

Abstract

This study was carried out to : (1) evaluate effectively of some plant extracts Basil (Ocimum basilicum L.) , Blue gum (Eucalyptus microtheca F.) , Myrtle (Myrtus communis L.), Oleander (Nerium oleander L.) and clove tree (Eugenia caryophylata Thunb) against the growth of Pythium aphanidermatum and In Vitro . (2) Determine the effective extract concentration on seedling damping - off caused by each pathogen on cucumber In Vivo . The results showed that ethanolic extract of the clove was superior over that of the other examined plants in preventing growth of pathogen. The growth of P. aphanidermatum was totally prevented at 500 and 1000 ppm clove ethanolic extract respectively. Portioning the clove ethanolic extract by various organic solvents indicated that the inhibitor (s) was mainly separated with diethyl ether and to less extract with ethyl acetate . Component(s) separated by the diethyl ether was inhibited the growth of pathogen at 1000 ppm. However , the component (s) separated by the ethyl acetate at 1000 ppm was cause 75% inhibition to P.aphanidermatum . On the other hand, total inhibition of P. aphanidermatum was obtained with powdered raw clove at 0.1 into Potato Dextrose Agar.