Possible correlation between O-antigen serogroup of Enteropathogenic / Enteroinvasive E.coli and co infection With Giardia G.lamblia and Entamoeba histolytica as a possible pathological strategy for acute diarrhea among children of Baghdad governorate

Abstract

Diarrheal diseases account for approximately 2 million deaths annually in children under the age of 5years. Disease and death caused by diarrhea is a global problem, but is especially prevalent in developing countries. The aim of the present study is to determine the possible role of E.coli mainly Enter pathogenic (EPEC) serogroup (O111, O55, O26, O86, O119, O127, O114, and O142) and Enteroinvasive E.coli (EIEC) serogroup (O124); Giardia lambleia and Entamoeba histolytica co infections in pathology of acute diarrhea among children's of Baghdad governorate. A total of one hundred seventy presented with diarrhea that proved through clinical investigation were enrolled in this study. After microbiological examinations, Sixty four children that infected with two pathogens were selectively enrolled in this study. The minimum age of infected children was 4 months while maximum age was 116 months. Thirty four (53.1%) % out of Sixty four children were males and females represent the rest 30(46.9%). Stool samples of patients were submitted for direct microscopical examination for detection of G.lamblia and E.histolytica using wet preparation technique. Gram staining technique was applied for demonstration of gram negative bacilli. Stool culture was done using MaCconky agar for determination of E.coli. API 20E system was used for biochemical characterization of E.coli .serogrouping of E.coli was done by direct slide agglutination technique in to four types (type1 EPEC serogroup ( O111,O55,O26 ), type 2 EPEC serogroup ( O86,O119,O127), type3 EPEC serogroup ( O125,O126,O128 ), type 4 EPEC/EIEC serogroup ( O114,O124,O142 ) using Specific trivalent antisera.