Comparison of the content of active compounds in cell suspensions and aqueous extracts of Rosmarinus officinalisL. with those in the Endophytic fungus Alternaria alternata

Abstract

The current study was carried out to detect the active compounds in both the aqueous extract of a Rosmarinus officinalisL. plant and the extract of cell suspensions and compare with the extract of the endophytic fungus Alternaria alternataisolated from the leaves of a R. officinalis plant. Callus was induced from the leaves of rosemary plants, and the highest induction ofcallus formed reached 100% when treated with a concentration of 1-Naphthaleneacetic Acid (NAA)2.0 mg. L-1, compared to the control treatment, which had an induction rate of 0%. the highest fresh weight of callus was 1.628 gm in the presence of NAA. The callus induced in the presence of NAA was charactrized by its fragile texture. cell suspensions were also obtained from it. fungus extract was superior in its content of active compounds to the aqueous extract and cell suspension cultures at 21 days. The total alkaloid content which were 6.65%, phenolic content were 117.25 mg.gm, flavonoid content were 104.35 mg.gm, total terpenoid content was 6.65%, total steroid content was 6.05% and the concentration of rosmaric acid was 154.7%. The results of the current study confirm the single receptor of endophytic fungi isolated from medicinal plants as one of the important sources of effective compounds