InhibitoryEffectoftheBiosynthesizedZnONanoparticlesbyMusaacuminataFruitsPeelsagainstPathogenicBacteriaIsolatedfromDiabeticFootUlcer

Abstract

ZincOxidenanoparticleswassynthesizedbygreenmethodwithzincnitrateasprecursorandalcoholicextractofMusaacuminatafruitspeelsas a reducingagent,colorchanging fromwhitetoyellowish -white, whichwasanindicationoftheformationofZnONPs.CharacterizationweremadebyUV-Visiblelightspectroscopy(Uv-Vis),X-RayDiffraction(XRD),Atomicforcemicroscopy(AFM)andFieldEmissionScanElectronMicroscope(FE-SEM),theresultsshowedthesharpUVpeakswereat374nm,XRDanalysisestimatedthecrystallitessizesin25.9nm,AFMwasusedtodeterminetheaveragesizeandformeofthenanoparticleswhichwas40,45,and50nm,FE-SEM analysis displayed average particles size were 75.6 nm. Clinical of 125 samples were collectedfromdiabetic footulcer frompatientsadmittedtoBaqubaTeachingHospitalduringbeginningof November 2021 to the end of January 2022, included isolation and diagnosis of 76 isolates ofEscherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. Antibacterial activity test was done of ZnO NPs. which was tested toward these isolates. Differentconcentrations of ZnO NPs. (12.5, 25, 50, 100, 200) mg/ml were examined against pathogenicbacteria,theresultsshowedthehighestdiameterofinhibitionzoneagainstE.coli,K.pneumoniae,P.aeruginosa,S.aureusandS.epidermidis