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Title: | Effecting of Ribosomal-Inactivating Proteins on Gene Expression of Cdr2 Gene in Pathogenic Candida Albicans Isolates |
Authors: | Yasser Muwafaq Mehdi Hadi Rahman Rasheed Al-Taai Rabab Majead Abed Ali Mohammed Sadeq |
Keywords: | fluconazol, CDR2, gene expression, RT-qPCR |
Issue Date: | 1-Jan-2024 |
Publisher: | University of Diyala |
Abstract: | Twenty isolates of Candida albicans were collected, during the study period from December / 2020 to February / 2021. For a group of 20 married women suffering from vaginal infections in Baqubah city. All the isolates were multidrug resistance against 5 antifungals from different groups according to the susceptibility test. The result of minimum inhibition concentration (MIC) for antifungal (fluconazole) values were between (256-64) μg/ml and MIC values for ribosomal inactivating proteins, between 325–5200 μg/ml . Conventional PCR technique was used to detect the CDR2 gene that is a molecular marker of a functional resistance for azoles antifungals group, the results inducted the presence of CDR2 gene 100%, it presented in the 20 isolates. By using Real-time PCR technique the CDR2 gene gave a low expression after treatment (32)μg/ml with sub MIC concentration of fluconazole with average folding 0.1once and after treatment 325 μg/ml with sub MIC ribosomal inactivating proteins concentration again of compared with control with average folding 0.6 in different degrees in two isolates were multi-resistant to antifungals. |
URI: | http://148.72.244.84/xmlui/handle/xmlui/15808 |
ISSN: | 2958-4612 |
Appears in Collections: | مجلة ديالى للعلوم الاكاديمية / Academic Science Journal (Acad. Sci. J.) |
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12-725ok.pdf | 1.06 MB | Adobe PDF | View/Open |
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