Study the Cytotoxic Effect of Nicotine Alkaloid Extracted from Nicotiana tabacum L. on Some Cancer Cell Lines

Abstract

Cancer is a major health problem and is a cause of death in the world. There are many approaches to its treatment, but they have many side effects. Recently, the researchers turned to find new methods for treatment by using natural sources such as plants that contain compounds that have anticancer effects. The current study aimed to determine the presence of Nicotine alkaloids in the extract of Nicotiana tabacum L. and study its cytotoxic effect on MCF7 (Michigan Cancer Foundation-7), and Hela (Henrietta lacks) human cancer cell lines. Dragendroff’s reagent and Gas Chromatography/ Mass Spectroscopy method is used to detect and measure the level of Nicotine. The cancer cell lines were treated with different concentrations of the extract. The results showed the effect of the extract on the growth of MCF7 that the lower rate of inhibition was 20.07% at (50 μg/ml), and the higher rate of inhibition was 96.67% at (800 μg/ml), but the inhibition rate of the extract on Hela was 34.87% at (50μg\ml), and the rate increased to 90.31% at (800 μg\ml).