TEST SOME LOCAL ISOLATES OF THE BACTERIOM Bacillus thuringiensis INSECT FIG MOTH Ephestia cautela (Lepidoptera: Pyralidae) REARED ON ARTIFICIAL DIET IN THE LABROTARY

Abstract

This research carried out in the laboratory of Technical Institute / Musayyib in 2011، has been tested six isolates local bacteria Bacillus thuringiensis on the first stage and the last of the larvae of a figs moth Ephestia cautela (Lepidoptera: Pyralidae) reared on the artificial diets ، while treated control with water only. ، And took the percentage of mortality results after 2، 5، 8 days since results showed that the highest mortality rate of 96.6% on the eighth day in each of the isolates DE 1-8 and NA 2-1 and DE 2-3 when Concentration 1.2 × 106 spore /ml. While less kill ratio at treatment SL 4-1 in the concentration of 1.2 × 104 spore /ml، amounting to 23.3%. isolation DE 2-3 achieved the highest rate at 72.2% at the concentration 1.2 × 106 spore /ml. within eight days، while the lowest rate was 23.3% when isolation SL 4 -1 Concentration 1.2 × 104 spore /ml. The higher efficiency relative to Bacillus thuringiensis bacteria in the mortality of the last instar larvae of the insect fig moth Ephestia cautella rate has reached 34.7% in isolation DE 2-3 focus 1.2 × 106 after the fifth day، while less relative efficiency rate was 6.8% when SL 4-1 in isolation Focus 1.2 × 104. The overlap between the concentration and the periods of isolation has achieved higher mortality in DE 2-3 at 46.8% at the concentration of 1.2 × 106 spore /ml after 5 days of treatment either at least it was 0.00% in isolation SL 4-1 at the concentration of 1.2 × 104 spore /ml after 2 days of treatment. As for the larvae that has not reached the stage of the pupae is observed that the lowest rate could not be reached 52.6 when isolation DE 2-3 focus 1.2 × 106 spore /ml . Which differed significantly from the treatment comparison، while the percentage larvae that could not is when isolation SL 4-1 in Focus 1.2 × 104 at 94%، which did not differ significantly from the treatment comparison، either adult that emerged from these pupae There was no significant effect on emergence percentage compared to the treatment of comparison where the lowest percentage rise of 88.8% when isolation AN 8-3 consecration 1.2 × 106 spore /ml while did not differ the rest of the other treatments for the of control mortality. The mortality efficiency was shown in the last larval instar compared to the first .