Evaluation of the possible role of HPV16, CMV and EBV in Cervical Carcinoma progression using In Situ Hybridization technique

Abstract

Background: Cervical carcinoma was considered as a major problem and life threaten of women; therefore it is worthy to study the association of Epstein–Barr virus and cytomegalovirus co-infection with human papillomavirus type 16 in uterine cervical carcinoma progression. Cervical carcinoma is known closely associated with human papillomavirus. Objective: To identify whether Epstein–Barr virus and cytomegalovirus play a co-factors role in the cervical carcinogenesis besides human papillomavirus type 16 infection. Patients and Methods: Current study included paraffin embedded sections from 50 cases of cervical cancer in Baghdad during the period from 2012 till 2014. They were examined for the presence of human papillomavirus type 16, cytomegalovirus and Epstein-Barr virus DNA using in situ hybridization technique. Results: This study showed that (33, 66%), (22, 44%) and (38, 76%) out of 50 cervical cancer specimens were positive to human papillomavirus type 16, cytomegalovirus and Epstein–Barr virus DNAs signals respectively by using in situ hybridization technique. Conclusion: The co-operation effects of CMV and EBV onto HPV16 might progress the oncogenesis of cervical carcinoma in female patients.