Genetic study of Staphylococcus Aureus Isolates from Different Environmental Source

Abstract

Background: Bacteria Staphylococcus aureus are from nurses and serious task because of the ability to cause various types of injuries at different locations of the body, and that pathogenesis has nothing to do with their ability to produce many virulence factors. Objective: To detection of virulence genes for Staphylococcus aureus isolates from different environmental source . Patients and Methods: The study involves isolation and ldentifieation of 60 bacterial isolate ,10 of them was identified as Staphylococcus aureus ,which is collected different environments (soil ,water) . This study focused on the pathogenic bacteria S.aureus which was isolated from the natural sources (soil ,water ) and was compared with S.aureus isolated from clinical samples isolates obtained from different clinical cases from Baquba General Teaching Hospital. Results: This study includes 10 samples of Staphylococcus aureus that isolated from soil and water, 10 samples of Staphylococcus aureus that isolated from hospital, identification it and detection of resistant genes by used Polymerase Chain Reaction technique. 4 isolates were identified genetical by 16srRNA gene and detection of resistant genesfemA, nucby used Polymerase Chain Reaction technique. The results of the genetic identification of the 16srRNA using polymerase chain reaction (PCR), revealed that all S.aureus isolates were positive (100%) and the results of resistant gene femA revealed that among 2 isolated belong to pathogenic S.aureus one isolate contains this gene (50%), whilst 2 isolates belong to S.aureus from the environment do not contain this gene (100%) . The PCR result for the nuc gene indicated that among each 2 isolated belong to pathogenic (clinical) S.aureus isolate one (1) isolate contain this gene (50%), whilst 2 isolates belong to S.aureus from the environment do not contain this gene (100%). Conclusion: Resistant gene femA get found in pathogenic S.aureus one isolate contains this gene , whilst isolates belong to S.aureus from the environment do not contain this gene , the nuc gene get found in pathogenic (clinical) S.aureus isolate one (1) isolate contain this gene , whilst isolates belong to S.aureus from the environment do not contain this gene