Kps M Detection in Acinetobacter Baumannii Isolated from Different Clinical Cases in Baghdad City

Abstract

Forty isolates of Acinetobacter baumannii were obtained from 200 samples. The isolates were collected from different cases including: - wounds, burns, stool, urine, sputum and blood samples. For the period between 1/9/2016 to 30/11/2016. from several hospitals in Baghdad city (Central Children's Hospital, Al Karama Hospital, Karkh General Hospital, Al-Ameen Medical City Hospital, Educational Labs, Baghdad Teaching Hospital, Child Protection Hospital, Burns and Wounds Hospital). Genotypic detection for virulence genes (kpsM) of Acinetobacter baumannii, the result revealed that the kpsM gene was present in 6 isolates (15%) of A. baumannii possessed the kpsM gene. The resulting packets with a molecular weight were 272 bp. Sequential analysis detection of kpsM showed that nine silent mutations that did not effect of the amino acid translation. While two mutations effected in the translation of amino acids as there was a change in path of translation of the protein, where the conversion of the amino acid (Lysine) to the amino acid (Asparagine) and converted the amino acid (Isoleucine) to the amino acid (Valine).