Effect of Colloidal Gold Nanoparticles Preparation Conditions on Viability of MCF-7 Breast Cancer Cells

Abstract

This study investigates the effects of changes of laser ablation energy parameters on the structural and optical properties of colloidal gold nanoparticles (AuNPs) prepared using pulsed Nd:YAG laser system at wavelength 1064 nm of highly purified solid targets of Gold noble elements plate in distilled water and studying the effects of colloidal AuNPs on the viability of MCF-7 cancer cell lines. Different laser ablation energies (500, 600, 700 and 800) mJ at a fixed number of laser pulses of (100) pulses were used to prepare colloidal AuNPs nanoparticles. The optical and surface morphological characteristics of AuNPs were studied using UV-Vis Spectroscopy, and Field Emission Scanning Electron Microscopy (FE-SEM). The results of UV-Vis absorption spectra showed an individual absorption peak in the visible region for the colloidal AuNPs solutions which appeared at (523-529) nm. Also, UV-Vis Spectroscopy results showed an increase in intensity of the absorption peaks and the concentrations of the materials.

The color of AuNPs solution was also changed from light to dark red with the increase in energy of the laser ablation. The results showed that the peaks of the surface Plasmon resonance peaks were shifted towards the longer wavelengths (converted to the red color). FE-SEM and TEM images analysis showed that most nanoparticle diameters were within the nanoscale. An increase in the average nanoparticle diameter was obtained with the increase of the laser ablation energy. The morphology of nanoparticles was almost spherical and homogenous with some agglomeration. The colloidal AuNPs showed concentration-dependent manner on the growth of MCF-7 breast cancer cells with IC50 value of about 12.98 mg/L.