Molluscum Contagiosum genome: phylogenetic analysis

Abstract

Molluscum Contagiosum virus (MCV), a double strand DNA virus that belongs to the Poxviridae family, MCV causes pearl disease. Seventy-five lesion samples were collected in Diyala province at dermatology clinics, from 30 September to 20 October 2019 from patients with Molluscum Contagiosum Virus after diagnosis by the specialist doctor, and further 25 samples were curreted frm skin as a control group collected from healthy. After viral genome extraction, specific primer was detected by conventional PCR then sequencing PCR then sequencing finaly registeration in NCBI and phylogenitic tree. Phylogenitic tree was generated to evlute the viral evalution and closest neighbour. The results of the PCR for control group (25 samples) were negative for all. From 75 samples only 7 samples were positive with specific bands at 393bp (9.3%) while 3 samples with specific bands at 393bp and nonspecific bands at 300bp (4%). Then samples with specific bands at 393bp that were divided into two groups according to similarity after sequencing. It was found that the matched rate was 100% when comparing each of these groups with the global isolates in NCBI. Two isolates were registered at NCBI with the accession number for the recorded isolates were LC520237 and LC520238.