Evaluation of markers CD4+, CD8+, IFN-γ in male smokers in diayla governorate / Iraq

Abstract

Background: Cigarette smoking habit is widely distribute in the whole world, obviously this bad habit responsible on many diseases which affect the general personal health of the smokers through its effect on the organs of the body in general and the immune system in particular. Objective:To determination of the smoking effect on some immunity parameters and compare the results with the non-smokers by using IFN-γ, CD4+and CD8+. Patients and Methods: 45 of smokers blood samples and 44 of non-smokers blood samples are collected. The levels of cellular of IFN-γ,CD4+and CD8+has been determined by using sandwich ELISA test. Results: The study included 45 smoker that have the average age of 29.60 ( ± ) 10.36 year, while the healthy non-smokers group have 44 person who's have the average age of 26.56 ( ± ) 9.09 years old. When comparing the level of CD4+T cells ,CD8+T and the level of IFN-γwith the number of cigarettes consumed per day, the class (20-1) was found to be the highest average of T cells.while (40-21) were less than average, with no significant difference. When comparing the level of CD4+T cells and the level of CD8+T cells with IFN-γwith smoking duration, the highest mean T-cell (10-6) years and the lowest mean (25-21). The results of the present study showed a statistically significant association between CD4+, CD8+and IFN-γmolecules. Conclusion: The general results of the tests on the effect of smoking on immunologic markers CD4+, CD8+and IFN-γassumed an increase in the ratio of the three indicators compared to the control group.