EFFECT OF BRASSINOLIDE, BENZYL ADENINE AND AUXINS ON in vitro PROPAGATION OF MYROB LAN PLUM AND GARNEM ROOTSTOCK

Abstract

This study was implemented in the tissue culture lab., College of Agriculture, Abu-Ghraib during the period Nov. 2011 to June 2014.. It was aimed to Effect of Brassinolide Benzyl Adenine and Auxins on In vitro propagation of Myroblan plum and Garnem Rootstock . The Sodium hypochloride concentration at 4.5% for 15 minutes was more effective on explants disinfestations. Percentages of contaminated explants decreased to 0.00% for Garnem and Myrob lan rootstocks respectively. In establishment stage , single nodal segments responded better than terminal shoots. MS medium supplemented with 0.4 mg / L NAA of outgrowth explants in the field for Garnem and Myrob lan rootstocks gave responded 60% and 40% respectively. But the medium provided with 0.3 mg / L IAA for Garnem and Myrob lan gave responded 40%, suitable for Myrob lan responded with 100% but Garnem gave responded rate 50% with 0.4 mg / L . In multiplication stage , MS medium supplemented with 0.5 mg / L BL + 2 mg / L BA was the best for number of shoot for Garnem and Myrob lan (15.62 and 17.38 shoots / explants) respectively . In Rooting stage , MS medium supplemented with 1.5 mg / L IBA or NAA gave 100% rooting percentage for Garnem and Myrob lan.