Inhibitory Effect of Myrtus communis L. and Syzygium aromaticum L. Extracts on the Growth of Staphylococcus aureus Isolated from Foot Ulcers of Diabetic Patients

Abstract

The study included 104 of two types of diabetic patients with foot ulcers. both male and female are involved. their age ranged (35-74) years, the first type of diabetes mellitus is insulin dependent and the second type is insulin nondependent. The results showed a significant difference (P<0.05) in infected males 75.96% compared with females 24.04%. The study has been performed at Baquba Teaching Hospital during the period from October 2016 to the end of March 2017. In this study, 35 isolates of Staphylococcus aureus have been isolated and diagnosed according to bacteriological and biochemical diagnostic criteria. The study showed that the hot aqueous extract of Myrtus communis and Syzygium aromaticum had strong effect at concentrations of (12.5,25,50,100,200 mg/ml) against the selected isolate of S. aureus with the diameter of inhibition zone (10.20,14.00,16.33,19.21, 22.27mm. respectively) for the first plant, while they were (9.80,12.31,15.56,19.21,21.22 mm. respectively) for the second plant. At the same concentrations mentioned above. the alcohol extract showed an inhibitory effect and the diameter of the inhibition zones were (8.23,12.31,14.21,18.33,20.31mm. respectively) for the first plant, while they were (13.00, 16.33,18.33 ,20.31,24.04mm. respectively) for the second plant. In comparison, the alcohol extract of Syzygium aromaticum showed the higher inhibitory activity against S. aureus. Antibiotic sensitivity performed on all bacterial isolates. twelve type of antibiotics were used, the isolates of bacteria revealed 100% sensitivity to both Vancomycin and Rifampin, while 100% resistance showed to Ampicillin and Penicillin G