Polymorphisms of Eselectin gene in the Arab Iraqi patients with Diabetes mellitus Type 2

Abstract

Diabetes - Type II (DMT2) is a group of metabolic diseases resulting from high blood sugar because of a defect in insulin secretion, insulin action, or both This study Conducted to evaluate serum level of factor E-selectin in patients with diabetes and its relationship to the Polymorphisms genetic gene E-selectin at the site A561C and assess the relationship between classical factor E-selectinfactors . The study included 100 male sample of Iraqi people. The first group (80) patients with diabetes type II and second group (20) of healthy as control samples. Was measured clinical indicators of blood serum for both groups included measuring the level of sugar in the blood and HbA1c and cholesterol and triglycerides and HDL and LDL and VLDL AthergenicIndex. Blood sugar and HbA1c showed significant differences between patients and control while the cholesterol and fat of all kinds showed no significant differences, except for fat, high-density non-significant differences compared to control patients. It was measured by the concentration of the adhesion molecule E-selectin in the patients' blood serum (66) and compared to control a person (20) individual using the ELISA device and results showed significant differences in patients than controls. Molecular study parts included (74) individual patients and (20) of individual control. It included PCR-RFLP technique. Electrophoresis results for Jene E-selectin at the site A561C showed the presence of alleles are A, C and the presence of three genotypes are, (AA, AC, CC ) and repeat compositions genetic (AA, AC, CC) with no significant differences in the patient group than controls . History allele a higher proportion of allele C in patients and control, and showed allele a no significant difference in control compared to patients using the test Fisher and relying on the Confidence Intervals (CI) and showed a preventive faction (PF) of the disease, and that the allele C showed a no significant difference in patients than controls using test Fisher depending on the Confidence Intervals (CI) and showed that the allele C was Etiological faction (EF). Repeat genotype AA difference is no significant in control compared to patients style of preventive faction (PF) of disease, showed a repeat genotype AC difference is no significant in patients than controls and appeared style of Etiological faction (EF) of disease, showed a repeat genotype CC no significant difference in patients than controls and Etiological faction (EF) of disease.