Comparison Study Among Some Methods That Used For Detection of Helicobacter pylori In Patient's With Gastrointestinal Disease

Abstract

The study aimed to comparison among some methods that used fordetection Helicobacter pylori in patient's with gastroduodenal disease and evaluate the beast method. The study samples collected from endoscopy unit in Baqubah Teaching Hospital for the period (29-9-2015 to 25-2-2016). They study included 30 case patients with gasteroduodenal disease also it included a study on (10) samples of apparently healthy individuals (control) as comparison group.The results showed the detection of Helicobacter pylori directly from biopsy by Polymerase Chain Reaction (PCR) recorded heights percent among other tests which are included: rapid urease test for biopsy, Rapid anti H. Pylori test (IgG) and bacterial culture. The percentage of detection H. Pylori in patients group by these four tests were 80%, 56.67%, 43.33%and 10%respectively. There were significant differences (P ≤ 0.001) among different tests. The present study concluded that thePCR technique is suitable for accurately detection of these bacteria directly from gastric biopsy specimens and the difficulty of culturing H. pylori from samples taken from patient's with gastroduodenal disease which handlers treatmentsof antibiotics and proton pump inhibitors )PPI(