HISTOPATHOLOGICAL AND PROPHYLACTIC EFFECT OF THE DANDELION ETHANOLIC EXTRACT AND LACTOBACILLUS ACIDOPHILUS ON PATHOGENIC BACTERIA in vitro AND in vivo STUDY.

Abstract

This study was designed to explore the pathological and prophylactic effect of the mix of Dandelion ethanolic extract with the bacterium lactobacillus acidophilus in vitro and in vivo using laboratory mice. Dandelion ethanolic extract, lactobacillus acidophilus and the mix of Dandelion ethanolic extract with the bacterium lactobacillus acidophilus were dealing in in vitro study against (Salmonella typhimurium, Pseudomonas aerogenosa , Staphylococcus aureus and Escherichia coli) using the concentration 100 mg/L for Dandelion extract, also dealing with the virulent bacteria in equal quantity and separately. Lactobacillus acidophilus had the highest inhibitory effect on the Pseudomonas aerogenosa, and the less inhibitory effect was by Dandelion extract, and the inhibitory effect of Dandelion extract on the pathogenic bacteria increased after mixed with lactobacillus acidophilus bacteria. Twenty four mice were randomly divided into six groups, each ghroup contain four animals. The first group infected with Pseudomonas aerogenosa and the dose 1x106 cfu orally for 48 hours. The second group infected with Pseudomonas aerogenosa with dose 1x106 cfu orally for two weeks. The third group infected with Pseudomonas aerogenosa and the dose 1x106 cfu orally for 48 hours, they treated with the mix of Dandelion ethanolic extract and lactobacillus acidophilus orally (0.3 ml/mice) for two weeks. The fourth group were treated with the mix of Dandelion ethanolic extract and lactobacillus acidophilus orally (0.3 ml/mice) for two weeks, and then infected with Pseudomonas aerogenosa and the dose 1x106 cfu orally for 48 hours. The fifth group administered Dandelion extract and concentration 100 mg/ml (0.3 ml/mice) orally for two weeks. The sixth group administered 0.3 ml/mice of normal saline as control group. The histopathological study showed pathological changes in the internal organs of the first and second groups that infected with Pseudomonas aerogenosa bacteria. The mix of Dandelion extract and lactobacillus acidophilus apparently has therapeutic effect more than prophylactic effect on the inhibition of Pseudomonas aerogenosa growth.