Effect of Crude alcoholic and alkaloids of plant Punica granatum(L.) in Mitotic index and cell line cancer

Abstract

This research was designed to study the effect of alcohol and alkaloids extract of Punica granatum in vivo on Lymphoid cell division of human blood in the equatorial phase also this study test toxic extracts in lymphatic cell in human used concentration 125 ,250, 500, 1000, 2000, 4000 mg\ml and compared with colchicine.The results showed that alcohol and alkaloids relatively have acute toxic increased 91% in 4000 mg\ml concentration with colchicien and. This study aimed to investigate the effect of the alcohol and crude alkaloids extracted from Punica granatum the on two malignant cell lines Hella and RD. Also this study included evaluation of the effect of these extracts on several cytogenetic parameters .The cytotoxicity of the alcohol extract and crude alkaloids extract was investigated on the cancer cells line, Hella and RD. Toxic effect for both extracts was indicated by rate of proliferation inhibition.The alkaloids extract showed the inhibition of Hella cell line at percentage 18.61-75.08% more than the alcohol extract 7.87- 50.50% at concentrations: 62.5-4000 mg/ml. The rate inhibition of alkaloids extract for RD cell line 10.48.-67.75 was higher than that of the alcohol extract 9.98-40.15 at the concentrations 62.5 and 4000mg/ml.