Flame Photometric and Molecular Absorption Determination of Metronidazole in pure form and Pharmaceutical preparations

Abstract

Simple, rapid and sensitive flame atomic emission spectrophotometric method is described for the determination of trace amount of Metronidazole Benzoate in pure and its pharmaceutical preparations. The method was based on the oxidation of Metronidazole Benzoate by potassium permanganate in alkaline solution to form an intense yellow soluble product and the intensity emission measured of potassium at emission line 766 nm using flame emission spectrophotometer. Beer's law is obeyed over the concentration range of 5-45 µg/mL, and relative standard deviation RSD% were (1.645, 1.705), and detection limits were (1.844 , 1.635 µg/mL), for tablets and injections respectively. The proposed method has been successfully applied for the determination of Metronidazole Benzoate in bulk drug and pharmaceutical formulations. The common excipients and additives did not interfere in these method.