Detection of Cystic Echinococcosis Antigen Concentration in Serum Patients

Abstract

Cystic Echinococcosis (CE) or Hydatidosis, a parasitic disease that caused by larvae of Echinococcus granulosus, is an important zoonosis endemic in large parts of the world and Iraq. Diagnosis of hydatidosis antibodies is based on immunodiagnostic methods (ELISA), along with radiological and ultrasound examinations, when treatment by chemotherapy and surgery.The objectives of the present study were to determine a specific antigen concentration -based ELISA technique for diagnosis of hydatidosis and compare it before and after surgery A total sample of 102 Iraqi subject from different hospitals were included in this study. They were distributed as 52 patients (43 females and 9 males, included the same group of patients study after 3-7 days surgery to remove hydatid cyst), and 50 persons healthy controls. Hyper immune-serum was raised against hydatid cyst fluid in rabbits. Anti-hydatid cyst IgG was purified by Ione exchange chromatography. The results show that increased circulating hydatid cyst antigen concentration before surgery 38.7µg/ml than antigen concentration after surgical removal hydatid cyst 25.7 µg/ml the difference was significant at P≤0.01. In the present study, the presence of circulating antigens in sera of hydatidosis patients by indirect ELISA[new kit] was detected hydatid cyst antigen in preoperative sera in 50 out of 52 (96.15%), the test demonstrated antigen in 48 out of 52(92.30%) postoperative sera