Investigation for the Alteration in Some Kinetics Parameters of Free and Immobilized Cellulase from Local Fungus Trichoderma viride

Abstract

Investigations were carried out to study the immobilization behavior of cellulase Trichoderma viride on polystyrene an-ion nature of the AM-21-A, and cat-ion exchange fiber VION KN1.Immobilization of cellulase change the values for Km which were 2.5x10-5 , 4.7x10-5 , 6.8x10-5 for free and immobilized enzyme on VION KN-1, AM-21-A respectively, and Vmax was 75,59,33 g/lmin-1mg- for free and immobilized enzyme on VION KN-1, AM-21-A respectively. The optimum temperature of the activity of the cellulase was affected by immobilization where the enzyme showed maximum activity at 50 ,60 ,70 C˚ for free and immobilized enzyme on VION KN-1, AM-21-A respectively, however, the immobilized cellulase showed a higher thermal stability, with respect to temperature, compared to the free cellulase.