Study The Prevalence of Giardia duodenalis and Detection IgM, IgG , IgA in Some Patients with Giardiasis

Abstract

This study was aimed to determine the prevalence of Giardia duodenalis from human stool samples during a period started from September 2012 to May 2013 in Al-Karkh region/Baghdad by using direct microscopic examination and to measure the concentration of immunoglobulin IgM, IgG, and IgA in serum of 50 infected patients by Enzyme Linked Immunosorbent Assay (ELISA). Results revealed, from 1194 human stool samples Giardia duodenalis was detected in 256 samples and the infection rate was 21.44%.Sex of the patients had significantly influence on the total infectivity rate, in males, females were 20.58% , 22.93% respectively. A significant effect of age on incidence of infection was noticed, the higher rate was recorded in children aged ≤ 10 years old 27.18% in comparison with 14.54% of patients aged ˃ 10 years old. The mean concentration antibodies specific for Giardia duodenalis in serum samples showed Significant differences (p< 0.05) between infected and non- infected humans and also among age groups of infected patients. The higher concentrations of IgM, IgG, and IgA were obtained in patients aged 2-12 years old in comparison with other age category .The conclusion of this study demonstrated significant differences in Giardia-antibodies levels between infected and non-infected individuals and among age groups. ELISA can be used for detection and differentiation between acute and chronic infection .