TOXIC EFFECT OF ALCOHOLIC AND ALKALINE EXTRACT OF Terbulues terrestris PLANT ON MITOCHONDIRAL DIVISIONS OF BONE MARROW CELLS AND TUMOR SIZE REDUCTION IN LABORATORY MICE

Abstract

This study was aimed to evaluate the effect of Terbulues terrestris fruit extraction on mitotic index and tumor HepG2 cell line (Hepatic cell line) inhibition in mice in different doses of alcoholic and alkaloids extraction (10, 20, 40, 80, 160) mg kg1- compared with colchicines methods by used injection methods. The results showed that alcohol and alkaloids relatively that a significant inhibition in mitotic index was recorded at dose (160 mg ml-1) which was the best in alkaloids extraction and in alcoholic extraction, increased 70% with colchicines and acute toxic effect in mice in respect to Lethal dose LD50 in mice (102 mg kg-1 and 98 mg kg-1), respectively in alcoholic and alkaloids extraction. The results of dose in alcoholic extraction (0.2, 0.4, 0.8 mg ml-1) which was injection intra peritoneum in 30 day, one dose every 48 hours in mice reduced the tumor 57.20 m3 in 1 mg ml-1 dose, but the alkaloids reduced the tumor 35.12 m3 in 0.8 mg ml1- compared with control 3508.16 m3 and 3855.80 m3, respectively. However the chronic toxicity of water extract at three different concentration and alkaloids reduced the tumor depend the time and concentration.