Effect of Inhibitors β-Lactamase on Recovery Effectiveness of Some β-Lactam Antibioticis Against Pseudomonas Aeruginosa

Abstract

Thirty-four samples with position Pseudomonas aeruginosa cultures isolated from bourns, wounds urinary tract infection and Otities media were collected from Baquba General Hospital during September-December 2010. The sensitivily of these isolates were tested against (16) antibiotics. The results showed that the highest resistances were for Amoxicillin, Ampicillin, CO-Trimoxazole and Nitroforautoin with 100%, while the lowest resistance was for Ofloxacin with 3% .The results of minimum inhibitory concentration (M.I.C) toward eleven antibiotics showed different range among isolates, some were able to resist high concentration of Ampicillin and Amoxicillin reach to 1024µg/ml, while others were inhibited by 2µg/ml of Ciprofloxacin. The isolates showed low sensitivity for combination Ampicillin-Sulbactam with 0%, while it shwed high sensitivity toward combination of Piperacillin-Tazobactam and Ceftazidime-Clavulanic acid 91.17, 100% respectively. The results of plasmid content was studied indicate that all isolates contain single large plasmid band, while the study of plasmid curing appear the plasmid loss at concentration 512 µg/ml of acridin orange.