STUDY OF EFFECT OF SPRAYING WITH ASCORBIC ACID, TOCOPHEROL, AND SILICON ON SOME BIOCHEMICAL AND MOLECULAR CHARACTERISTICS OF DATE PALM OFFSHOOTS GROWN IN SALINE ENVIRONMENT

Abstract

This study was carried out in a private orchard in Basrah governorate Al- Hartha area – Al – Mashab area during the tow growing seasons 2017 and 2018 inorder to study the effect of foliar spray with the antioxidants Ascorbic acid, tocopherol and silicon on certain aspects of, biochemical and molecular characteristics of date palm leaves offshoots cv. Barhi grown in saline environment .The results showed that spraying with ascorbic acid at 450mg.L-1 caused a significantly incrase nitrogen concentration in the leaves for the first and second seasons and for the potassium element for the second season only, compared to The control treatment , which recorded the lowest values of nitrogen and potassium . The sam treatment recorded the lowest sodium concentration in the leaves and the highest percentage of K/Na for the second season only. The treatment was superior to tocopherol concentration of 300 mg. L-1 a significantly in the concentration of phosphorus in the leaves and for both seasons reached, compared to the control treatment which recorded the lowest values. The silicon spray treatment was recorded at 600 mg . L-1 gave the highest percentage of K/Na ration in the first season was measured by control treament with the lowest values. The treatments of ascorbic acid and tocopherol affected the gene expression process resulting in a number of protein bundles whose molecular wights ranged from 29.32- 82.00 KD. The treatment of spraying for time was significantly superior to the treatment of two sprays in the studied traits. The results showed that the interaction between both factors ascorbic acid at a concentration of 450 mg. L-1 and the number of sprays four times is the most significant effect in the characteristics under study.