EVALUATION OF HUMORAL IMMUNE RESPONSE TO ASLIME PRODUCING Staphylococcus aureus MASTITIS VACCINE IN AWASSI EWES

Abstract

This study aims to evaluate the humeral immune response to a slime producing Staphylococcus aureus mastitis vaccine through the preparing two vaccines (Live attenuated and killed vaccines) from a slime producing Staphylococcus aureus bacteria isolated from Awassi ewes with mastitis. The first part of study was carried out to isolate the slime producing Staphylococcus aureus bacteria from many lactating Awassi ewes located around Baghdad. The second part included preparation of live attenuated and killed vaccines from isolated S.aureus. Safety and sterility testes were done on vaccines preparation. In the immunization study, fifteen Awassi ewes were randomly divided into three equal groups. Group-A:- Ewes were inoculated with one ml of a live attenuated vaccine (containing 1x10¹º CFU) S/C near supra mammary lymph node, twice at 2 weeks intervals. at first month of lactation. Group-B:- Ewes were inoculated with killed vaccine twice, two weeks interval, at the same dose, route and time as previously described in group-A. Group-C:- Served as control. Clinical examination had been carried out in all animals pre and post vaccination. Moreover Serum and milk whey was obtained for measuring antibody by Elisa test, Animals show normal clinical signs pre vaccination. Nevertheless, moderate increase in body temperature, respiration, pulse rates in vaccinated groups, however the control group show normal systemic reaction pre and post vaccination. The vaccines induced humoral immunity, group-A revealed higher significant (P≤0.05) antibody titers in serum (0.136± 4.74) and milk whey (2.55±0.15( as compared with group-B which exhibited antibody titers (3.68± 0.153) in serum and whey attained at day 42 post- vaccination and thereafter, a decline in titers was observed towards the termination of the study. While control group showed low titers of antibody.