THE EFFECT OF DENSITIES CULTURE OF CELL SUSPENSION PLANT Withania somnifera L. IN CELL PLATING AND ARRAY METHOD IN THE FORMATION OF CALLUS PRIMORDIAS

Abstract

This study succeeded in establish cell suspension derived from callus hypocotyle Withania somnifera L. seedlings, induced in MS solid medium supplemented with 3.0 mg l-1 2,4-D Intertwined with 0.5 mg.L-1 Kin. Detection culture different densities (1.0, 1.52, 1.79, 2.03) ×104 cell cm-3 of these suspensions of plating and array method of agar-solidified MS medium in the presence of concentrations 3.0 mg l-1 2,4-D + 0.5 mg l-1 Kin. Culture method by plating was exceeding the array method in the number of cellular colonies. It has reached the 27.600 colony dish-1 at the culture density 2.03 ×104 cell cm-3 compared with the initiation density which amounted to 8.125 colony dish-1 in plating method, while the number of these colonies by array method was 21.400 colony dish-1 using the same culture density compared with the initiation density which amounted to 10.400 colony dish-1. Moral high density excelled significantelly on the rest of the densities opals giving 58 callus primordial after 20 days of culture of cells suspension using plating method, and giving 6 callus primordial after 25 days of culturing on cell suspension when array method was used.