STUDY THE INHIBITORY EFFECT OF OMPERAZOLE ON EFFLUX PUMPS FOR CIPROFLOXACINE AND NORFLOXACINE ON SOME BACTERIAL ISOLATE FROM PATIENT

Abstract

The inhibitory effect of omperazole on drug efflux pumps for decrease of antibiotic resistance in some isolates included(E.coli, Enterobacter, Staphylococcus aureus., Pseudomonas aeruginosa) to ciprofloxacin and norfloxacne.The minimal inhibitory concentration(MIC) of ciprofloxacin and norfloxacin were determined by using tow fold dilution method on muller hinton agar. The MIC of ciprofloxacine was 256μg/ml,64μg/ml, In E.coli and Enterobacter respectively and 512μg/ml in S.aureus and Ps. aeruginosa. the MIC values of norfloxacin was 512μg/ml in E.coli and Ps.aeruiginosa and 1024μg/ml in S. aureus and 256μg/ml in Enterobacter . On the other hand the MIC of omperazole alone was tested there was no effect on pathogenic bacteria. The MIC of ciprofloxacin and norfloxacion were determined after added of 100mg/ml of omperazol on each of concentration .the MIC of ciprofloxacin became 32μg/ml in E.coli and Enterobacter and 128μg/ml,64μg/ml for S.aureus and Ps.aeruginosa. while the MIC of norfloxacin after added omperazole was 128μg/ml to E.coli , 64μg/ml to Enterobacter and 256μg/ml for S.aureus and Ps.aeruginosa