The Tissue Culture Media Supplement to Improve In Vitro Embryos Production in Ewes: A Review

Abstract

The Iraqi sheep production is important economically when increase interest skin, wool, milk and meats. To increase the in vitro embryo production must interest the estrous synchronization, superovulation and in vitro embryo production (IVEP) in sheep. Several successes has been in recent years in biotechnology reproduction in farm animals especially in sheep,but several limitation of IVEP in each stage procedure, so it less than ambitious. For this estimated 30-60 % from the oocyte culture reach to metaphase II in maturation stage , 20-35% from mature oocytes becoming zygote after fertilization stag and 10-25% from embryo development to blastocyst stage . Also the embryo production in vitro has low implantation rate when embryos transfers because has low quality than in vivo. For tis require development and interest of tissue culture media supplement to improve all embryo production stage and more laboratories used one or more different tissue culture supplement to improve embryo production in farm animals. The media supplement with antioxidant, hormones, fetal calf serum, follicular fluid and antibiotics, mostly all these material addition to culture media to improve embryo production in sheep. Conclusion the supplement tissue culture media to in vitro embryo production in sheep have positive effect advantage to increase maturation, fertilization and culture embryo in the laboratory experiment.