Preparation Zno Nanoparticles with Different Concentration byLaser Ablation in Liquid and Their Use in Anti Bacterial Activity

Abstract

The study included the preparation of ZnO nanoparticles using (P.L.A.L.) technology, as their optical properties were improved. The effect of the number of pulses (400, 500, 600) made from distilled water (DW) as a growth medium was examined using an Nd: Yak laser. The wavelength used (1064 nm), the ablation energy (600 mj), the repetition rate (1 Hz), the diameter of the laser beam at the focal center (2 mm) of the target, and the distance between the target and the lens (8 cm). Tests were conducted and the results showed ( FE-SEM) studied the surface morphology of the prepared material, and average size diameters ranging from (80.29 - 35.62 nm) were obtained for ( ZnONPs ). The results of the FTIR examination showed that pure functional groups with different patterns and densities of absorption bands form zinc dioxide nanoparticles. It was shown that absorption appears for the beam near the wave number (478.3463 cm-1 ) due to the coupling vibration (Zn-O), and the absorption band appears near the wave number (1660.71 cm-1) due to the vibration of the (H-O) beam. UV spectroscopy measurements showed a clear absorption peak at the wavelength (375 - 380 nm ). In biological application, nanoparticles have shown bacterial inhibition by acting as antibiotics against bacterial strains (S. epidemidis, P. aeruginosa, and E.coil).