Please use this identifier to cite or link to this item: http://148.72.244.84/xmlui/handle/xmlui/16053
Title: Phenotypic Identification and Molecular Characterization of Aspergillus Niger, Fumonisin and Ochratoxin Genes from Otomycosis in Domestic Cats with Special Emphasis to Underlying Risk Factors
Authors: Rusul Muayad Hadi
Keywords: Aspergillus niger
Fumonisin
Ochratoxin
otomycosis
cat
Issue Date: 1-مار-2025
Abstract: The current study aims to isolation of Aspergillus niger from external ear of cats, molecular characterization of A. niger, fumonisin and ochratoxin by conventional PCR and detection of risk factors. A total of 106 external ear swabs were taken from domestic cats and cultured on Sabouraud dextrose agar. Positive fungal growth was identified by conventional PCR using species-specific PCR primer pairs for A. niger (NIG1) and (Cmd A)—production of fumonisin and ochratoxin confirmed by PCR using Fum 1 and PKS specific primers. The total of (20.75 %) swabs were A.niger positive by culture and PCR using ( NIG1) and (Cmd A) primers. Cats with age (2-6) months were frequently infected followed by those with (12- 16) months. Males represent (62.26%) while female represent (37.73%). A total of (15.09%) males were infected with A.niger versus (5.66%) for females . Females appear to be at risk of getting A. niger infection at (1.813) time than males. No significant correlation was reported between age, sex, body weight; breed; hair length; ear cleaning interval; bathing frequency; season and infection with A. niger. Fumonisin was detected in (41.17%) of A. niger isolates while ochratoxin was not detected.
URI: http://148.72.244.84/xmlui/handle/xmlui/16053
ISSN: 2410-8863
Appears in Collections:مجلة ديالى للعلوم البيطرية / Diyala Journal for Veterinary Sciences (DJVS)

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