Please use this identifier to cite or link to this item:
http://148.72.244.84/xmlui/handle/xmlui/16053
Title: | Phenotypic Identification and Molecular Characterization of Aspergillus Niger, Fumonisin and Ochratoxin Genes from Otomycosis in Domestic Cats with Special Emphasis to Underlying Risk Factors |
Authors: | Rusul Muayad Hadi |
Keywords: | Aspergillus niger Fumonisin Ochratoxin otomycosis cat |
Issue Date: | 1-مار-2025 |
Abstract: | The current study aims to isolation of Aspergillus niger from external ear of cats, molecular characterization of A. niger, fumonisin and ochratoxin by conventional PCR and detection of risk factors. A total of 106 external ear swabs were taken from domestic cats and cultured on Sabouraud dextrose agar. Positive fungal growth was identified by conventional PCR using species-specific PCR primer pairs for A. niger (NIG1) and (Cmd A)—production of fumonisin and ochratoxin confirmed by PCR using Fum 1 and PKS specific primers. The total of (20.75 %) swabs were A.niger positive by culture and PCR using ( NIG1) and (Cmd A) primers. Cats with age (2-6) months were frequently infected followed by those with (12- 16) months. Males represent (62.26%) while female represent (37.73%). A total of (15.09%) males were infected with A.niger versus (5.66%) for females . Females appear to be at risk of getting A. niger infection at (1.813) time than males. No significant correlation was reported between age, sex, body weight; breed; hair length; ear cleaning interval; bathing frequency; season and infection with A. niger. Fumonisin was detected in (41.17%) of A. niger isolates while ochratoxin was not detected. |
URI: | http://148.72.244.84/xmlui/handle/xmlui/16053 |
ISSN: | 2410-8863 |
Appears in Collections: | مجلة ديالى للعلوم البيطرية / Diyala Journal for Veterinary Sciences (DJVS) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Phenotypic identification and Molecular Characterization of Aspergil-lus niger,.pdf | 773.44 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.