Clinical compatibility Between Negative Stains, Quick Gram Chromo-trope, Gram And Giemsa Staining Techniques For Detection Of C.Parvum Infection In Children Under 5 Years

Abstract

Aim : To investigate the agreements between chromotrope gram hot, gram, Giemsa , nigrosin and mala-chite green staining techniques for detection of C.Parvum infection in children under 5 years in Baqubah-Diyala province Methods :One hundred diarrheic children under 5 years were included .Stool samples were examined after stained with hot Ziehl –Neelsen , Chromotrope Gram Hot; Gram ,Giemsa, Nigrosin and malachite Green. Results : ZN hot and all alternative staining techniques were identical in diagnosis of (26%) negative C.Parvum infection among diarrhea cases in children .A total of (9%) with low score in chromotrope Gram Hot ,(10%) moderate and (19%) have heavy score in both techniques. Moderate agreement (kappa =0.514),was reported between ZN hot and chromotrope Gram Hot for diagnosis of C.Parvum oocysts in children . A total of (11%) of cases have low score in ZN hot and Gram Hot staining , (8%) were moder-ate and (19%) have heavy score in both techniques. Moderate agreement (kappa =0.525),was reported be-tween ZN hot staining and Gram Hot . A total of (12%) of cases have low score in ZN hot staining and Giemsa; (10%)were moderate and (18%) have heavy score in both techniques. Moderate agreement (kappa =0.552 ),was reported between ZN hot staining and Giemsa for diagnosis of C.Parvum oocysts . A total of (9%) of cases have low score in ZN hot staining and nigrosin, (13%) were moderate and (28%) have heavy score in ZN hot staining and nigrosin. Moderate agreement (kappa =0.665),was reported be-tween ZN hot staining and Nigrosin. A total of (10%) have low score in ZN hot staining and malachite green ,(8%) were moderate and (19%) have heavy score in both techniques .Moderate agreement (kappa =0.515),was reported between ZN hot staining and malachite green. Conclusion : All Alternative stains for Hot ZN( Chromotrope Gram Hot; Gram ,Giemsa, Nigrosin and malachite Green have identical specificity (100%) for diagnosis of negative C.Parvum among diarrhea cases in children . Moderate agreement was reported between ZN hot staining and all alternative stains (Chromotrope Gram Hot ; Gram Hot ,Giemsa , Nigrosin and Malachite Green) for diagnosis of C.Parvum oocysts in children which represent good alternatives in rural areas and low income countries