Isolation and Identification of some Terrestrial invertebrate's bacteria from Baghdad, Al-Jadriya

Abstract

This study aimed to isolate and culture the bacteria from surfaces of some terrestrial healthy invertebrates from Al-Jadriya, Baghdad. Four major phyla of terrestrial invertebrates were obtained, Arthropoda genus Porcellio sp. and Julus sp., Mollusca genus Monacha cantiana, Nematoda, genus Seinura sp. and Annelida genus Pristina sp., the bacteria were characterized by using selective culture media and biochemical tests. The surface associated bacteria strains were identified as Alteromonas putrefaciens , Bacillus pumillus , Citrobacter freundi , Actinomyces sp. , Bacillus azotoformans , Serratia marcescens , Yersinia sp. , Proteus vulgaris , Pasteurella pneumotropica , Edwardsiella tarda , Enterobacter aerogene and Micrococcus sp. , The biological activities of the isolated microorganisms were checked against some of the important pathogenic bacteria like Escherichia coli and Staphylococcus aureus by Agar well diffusion assay. We found that Porcellio sp., Julus sp. and M. cantiana which refer to (Arthropods and Mollusca) have higher biological activity isolated from their surface than other bacterial invertebrates compared with other isolates which were refer to, Alteromonas putrefaciens , Bacillus azotoformans and Pasteurella pneumotropica respectively