Study toxic effect for extraction alcoholic and aqueous to plant Isatis tinctoria in same cell line

Abstract

This study aimed to investigate the effect of the aqueous and crude alcohol extracted from the leaves of Isatis tinctoria on two malignant cell lines Hela and RD. Also this study included evaluation of the effect of these extracts on several cytogenetic parameters. The cytotoxicity of the aqueous extract and crude alcohol extract was investigated on the cancer cells line, Hella and RD. Toxic effect for both extracts was indicated by rate of proliferation inhibition. The alcohol extract showed the inhibition of Hela cell line at percentage (19.17% - 93.03%) more than the aqueous extract (8.95% - 75.69%) at concentrations: 62.5-8000 g ml-1. The rate inhibition of alcohol extract for RD cell line (11.48% - 77.87%) was higher than that of the aqueous extract (9.60%-71.14%) at the concentrations 62.5 and 8000 g ml-1. Both extracts (alcohol extract and aqueous extract) showed almost the same effect at the concentration 8000 g ml-1.