Isolation and Characterization of PAP a Lytic Bacteriophage Specific for Pseudomonas aeruginosa

Abstract

There has been a frightening increase in drug-resistant strains of Pseudomonas aeruginosa as a result; there has been a repeated attention in alternative antimicrobial treatments, as bacteriophages. Current study was included isolation and characterization of a lytic phage specific to Pseudomonas aeruginosa (which we designed as PAP referred to a Pseudomonas aeruginosa specific phage) from sewage water of General Baqubah Hospital treatment plant. In this study, a phage PAP was isolated and titrated, using double layer agar technique. and determination of the latent periods and burst size of phage PAP then determines their stability to some of physical and chemical condition (temperature, UV light, Alcohol, chloroform and isopropanol exposure).The results detected a lytic phage in contradiction of an antibiotic-resistant Pseudomonas aeruginosa, the stock titer about 8×109 PFU(plaque forming units )/ml. In phage PAP growth cycle, the latent period was 20 min, a burst size was 126 PFU/ infected cell. We found that the phages PAP stable at 4°C and 37°C while they declined quickly after boiling for 10-20 min. unaffected in the presence of chloroform in different concentration, affected by 10% isopropanol, more affected by 70% alcohol