Lactobacillus Acidophilus as Antibiofilm Formed by Staphylococcus Aureus Invitro

Abstract

Background: The lactobacilli are well known to have a positive effect on human health. These bacilli, which are formed the largest part of the microbiology natural, that characterized by its ability to inhibit the growth of bacteria through the production of various antimicrobial materials such as bacteriocins and biosurfactant, thus preventing the formation of biofilm by lactobacilli, and Staphylococcus aureus for examples of bacteria that have the ability to produce a biofilm. Objective: To determine biofilm production ability by Staphylococcus aureus isolates and evaluate effectiveness of Lactobacillus acidophilus to elimination of planktonic Staphylococcus aureus and their biofilm producers, in vitro. Methods: This study was carried out for the period December 2012 to April 2013 in Ramadi Teaching Hospital in Ramadi City. Fourty isolates of Staphylococcus aureus were isolated from blood, urine, surgical wounds, and intravascular catheters. All specimens were identified using biochemical tests, and they were tested for biofilm production by using Microtiter-plate method. Also, used to study the ability of Lactobacillus acidophilus supernatant to inhibit biofilm produced by Staphylococcus aureus, and used L. acidophilus supernatant to inhibit planktonic S. aureus (S2,S7,S11,S12,and S19) which are highest biofilm produced , in vitro. Results: Fourty Staphylococcus aureus were biofilm produced and distributed in to 20 (50%), 15 (37.5%), and 5(12.5%), our result showed that inhibitory effect of Lactobacillus acidophilus supernatant on planktonic S. aureus (S2, S7, S11, S12, and S19). Significant differences (P<0.01) were found between pre and post treatment of Staphylococcus aureus biofilm with Lactobacillus acidophilus supernatant. Conclusion: Staphylococcus aureus has high ability to produce biofilm, and the Lactobacillus supernatant eradicated planktonic Staphylococcus aureus and their biofilms remarkably in vitro.