Genotyping Of Polyomavirus BK For VP1 Gene Using Gene Specific Phylogenetic Trees Among Nephropathy Patients In Diyala Governorate

Abstract

Background BK virus-associated nephropathy (BKVAN) is an important reason for missing graft in kidney transplants Aims This paper emphasizes exploring the genetic polymorphisms of two BK polyomavirus samples isolated from the blood of patients with nephropathy. Methods A total of 170 blood plasma samples were collected from (130) nephropathy patients and (40) healthy blood donors. All participants were subjected to serological testing using ELISA to detect antiBKV IgM in the blood. positive antiBKV IgM samples were used for the detection of BKV DNA By RT-PCR. Two samples with the highest purity and concentration of DNA, were used to determine the genetic polymorphisms of two BK polyomavirus . Results: Only 20/130,( 15.38%) specimens of nephropathy patients have positive BKV IgM and all healthy group was negative. A total of (40%) was positive in RT-PCR for BKVP1gene and BKtyp4 primer. The genetic polymorphisms was found that the investigated samples were clearly incorporated within the clade of serovar IV in close phylogenetic positions to many strains isolated from Kuwait with no phylogenetic distances among them. Conclusions BK polyomavirus-VPI amplicons could be used as markers to differentiate between BK viruses. This study emphasizes the significance of genetic variation in comprehending the molecular epidemiology and evolutionary dynamics of BK polyomavirus, which may affect the phylogenetic framework and geographical tracking of the virus currently under investigation.