Study The Effect of Crude Oil in Some Physiological Parameters in Oscillatoria chlorin

Abstract

The effect of different concentration of crude oil had been examined upon Oscillatoria chlorina, it was depended upon measuring Growth rate as optical density, Chlorophyll, Phycocyanin and Protein concentration as an Physiological activities indicator of crude oil effects on Oscillatoria chlorina, in addition to morphological changes. The growth optical density in the control sample in the day 30 was (0. 43), while it was (0.3) & (0.18) at 0.25% & 0.5% crude oil respectively, and the growth inhibited under effect of concentration of crude oil ≥ 1%. A 40% and 68% of chlorophyll concentration was reduced under the effect of crude oil treatment 0.25% & 0.5% respectively. as well as protein concentration was reduced to 33% and 85% under the effect of 0.25% 0.5% crude oil respectively. Also, the growth, chlorophyll concentration, phycocyanin pigment and protein concentration were totally reduced under influence of crude oil 1%&2%. As well as the absorption spectrum infrared by FTIR show there were absence of methyl group (CH3) at (2920 cm-1 ) & (2850 cm-1 ), and this is could be due to the activity of Oscillatoria chlorine and also could be due to its consumed by this species. Morphologically, there were changing in the filament form to be tapering when it grew under the effect of crude oil