ROLE OF PLATELETS RICH PLASMA AND PLATELETS RICH FIBRIN MATRIX IN TREATMENT OF CHRONIC WOUNDS IN BUCKS

Abstract

The aims of this study was to evaluate the effects of platelet-rich plasma gel (PRP) and platelet-rich fibrin matrix (PRF) application on the induced open chronic wounds in bucks by clinical and biomechanical estimation. Twenty – four adult bucks were used in this study. Skin and subcutaneous tissues were removed to make four square full-thickness skin wounds (4×4 cm) on the dorsal sides of the back of each animal (two wounds on each side), 10 cm apart after preparation of the area in routine surgical manner one for treatment group and second as control group, by daily scratched to interrupt healing process continuation to prolong inflammatory reaction to form chronic wound. This surgical procedure was continued for eight week to ensure the chronicity state of these wounds, then they were treated by PRP gel in first group (n=12), while PRF matrix was used in second group (n=12). PRP gel and PRF matrix were prepared from whole blood at time of treatment, with leave two wounds of each animal as control for treated type. Healing process was evaluated clinically by measuring wound size of both treated and control groups, and throughout experimental time and biomechanical assessment of healing wounds in 45 day post operation. The healing process of treated wounds showed significant variation in wound’s dimensions of both treated groups in comparison to their control group without significant in between them, while the biomechanical evaluation indicated increasing in tensile strength of both treated groups in comparison to control group. The conclusions of this work is the continuous mechanical irritation of wounds site may lead to form chronicity state for the effected wounds and the application of PRP or PRF may improve healing process and increased wound tensile strength without any adverse effect, and biomechanical assessment of wound tensile strength of PRP and PRF groups within same consequences and provided a good indication of wound healing process.